ella simpleplex tm nfl assay kit Search Results


ella simpleplex tm nfl assay kit  (Bio-Techne corporation)
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Bio-Techne corporation ella simpleplex tm nfl assay kit
Ella Simpleplex Tm Nfl Assay Kit, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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commercial assay or kit simpleplex cartridge  (Protein Simple Inc)
90
Protein Simple Inc commercial assay or kit simpleplex cartridge
Commercial Assay Or Kit Simpleplex Cartridge, supplied by Protein Simple Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immunoassay kit protein simple ella simpleplex human pcsk9  (Protein Simple Inc)
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Protein Simple Inc immunoassay kit protein simple ella simpleplex human pcsk9
Immunoassay Kit Protein Simple Ella Simpleplex Human Pcsk9, supplied by Protein Simple Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ella simpleplex il1β, tnfα, mcp1/ccl2  (Protein Simple Inc)
90
Protein Simple Inc ella simpleplex il1β, tnfα, mcp1/ccl2
Repetitive IOP spikes induce the activation of inflammatory biomarkers and RGC dysfunction and loss. ( A ) Experimental paradigm of the spiking OHT (spOHS) model and steady OHT (stOHT) control; ( B ) qRT-PCR data on relative changes in transcript abundances of inflammasome pathway genes in retinas. Mean fold change vs. naïve ± SE, * p < 0.05, n = 3–5. ( C ) Intravitreal IL1-β measured at 6, 12, and 24 h after spOHT (red) and stOHT eyes (OD, blue). Mean ± SE, * p < 0.05, n = 5. Negative control: Casp1 −/− eyes (Cs1KO). ( D ) Quantification for HIF1a fluorescent labeling in retina wholemounts from eyes exposed to sham, stOHT, spOHT, or IR. Mean percentile change vs. sham ± SE, ** p < 0.05, n = 5.
Ella Simpleplex Il1β, Tnfα, Mcp1/Ccl2, supplied by Protein Simple Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ella simpleplex il1β, tnfα, mcp1/ccl2/product/Protein Simple Inc
Average 90 stars, based on 1 article reviews
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elisa kits mouse il-1β  (R&D Systems)
90
R&D Systems elisa kits mouse il-1β
Repetitive IOP spikes induce the activation of inflammatory biomarkers and RGC dysfunction and loss. ( A ) Experimental paradigm of the spiking OHT (spOHS) model and steady OHT (stOHT) control; ( B ) qRT-PCR data on relative changes in transcript abundances of inflammasome pathway genes in retinas. Mean fold change vs. naïve ± SE, * p < 0.05, n = 3–5. ( C ) Intravitreal IL1-β measured at 6, 12, and 24 h after spOHT (red) and stOHT eyes (OD, blue). Mean ± SE, * p < 0.05, n = 5. Negative control: Casp1 −/− eyes (Cs1KO). ( D ) Quantification for HIF1a fluorescent labeling in retina wholemounts from eyes exposed to sham, stOHT, spOHT, or IR. Mean percentile change vs. sham ± SE, ** p < 0.05, n = 5.
Elisa Kits Mouse Il 1β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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commercial assay or kit draq7  (Biostatus)
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Biostatus commercial assay or kit draq7
Repetitive IOP spikes induce the activation of inflammatory biomarkers and RGC dysfunction and loss. ( A ) Experimental paradigm of the spiking OHT (spOHS) model and steady OHT (stOHT) control; ( B ) qRT-PCR data on relative changes in transcript abundances of inflammasome pathway genes in retinas. Mean fold change vs. naïve ± SE, * p < 0.05, n = 3–5. ( C ) Intravitreal IL1-β measured at 6, 12, and 24 h after spOHT (red) and stOHT eyes (OD, blue). Mean ± SE, * p < 0.05, n = 5. Negative control: Casp1 −/− eyes (Cs1KO). ( D ) Quantification for HIF1a fluorescent labeling in retina wholemounts from eyes exposed to sham, stOHT, spOHT, or IR. Mean percentile change vs. sham ± SE, ** p < 0.05, n = 5.
Commercial Assay Or Kit Draq7, supplied by Biostatus, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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peptide, recombinant protein interferon gamma  (Boehringer Ingelheim)
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Boehringer Ingelheim peptide, recombinant protein interferon gamma
Repetitive IOP spikes induce the activation of inflammatory biomarkers and RGC dysfunction and loss. ( A ) Experimental paradigm of the spiking OHT (spOHS) model and steady OHT (stOHT) control; ( B ) qRT-PCR data on relative changes in transcript abundances of inflammasome pathway genes in retinas. Mean fold change vs. naïve ± SE, * p < 0.05, n = 3–5. ( C ) Intravitreal IL1-β measured at 6, 12, and 24 h after spOHT (red) and stOHT eyes (OD, blue). Mean ± SE, * p < 0.05, n = 5. Negative control: Casp1 −/− eyes (Cs1KO). ( D ) Quantification for HIF1a fluorescent labeling in retina wholemounts from eyes exposed to sham, stOHT, spOHT, or IR. Mean percentile change vs. sham ± SE, ** p < 0.05, n = 5.
Peptide, Recombinant Protein Interferon Gamma, supplied by Boehringer Ingelheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cytokine magnetic magpix 25 plex panel invitrogen magpix platform commercial assay  (Thermo Fisher)
86
Thermo Fisher human cytokine magnetic magpix 25 plex panel invitrogen magpix platform commercial assay
Repetitive IOP spikes induce the activation of inflammatory biomarkers and RGC dysfunction and loss. ( A ) Experimental paradigm of the spiking OHT (spOHS) model and steady OHT (stOHT) control; ( B ) qRT-PCR data on relative changes in transcript abundances of inflammasome pathway genes in retinas. Mean fold change vs. naïve ± SE, * p < 0.05, n = 3–5. ( C ) Intravitreal IL1-β measured at 6, 12, and 24 h after spOHT (red) and stOHT eyes (OD, blue). Mean ± SE, * p < 0.05, n = 5. Negative control: Casp1 −/− eyes (Cs1KO). ( D ) Quantification for HIF1a fluorescent labeling in retina wholemounts from eyes exposed to sham, stOHT, spOHT, or IR. Mean percentile change vs. sham ± SE, ** p < 0.05, n = 5.
Human Cytokine Magnetic Magpix 25 Plex Panel Invitrogen Magpix Platform Commercial Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chemical compound, drug methocult gf  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc chemical compound, drug methocult gf
Repetitive IOP spikes induce the activation of inflammatory biomarkers and RGC dysfunction and loss. ( A ) Experimental paradigm of the spiking OHT (spOHS) model and steady OHT (stOHT) control; ( B ) qRT-PCR data on relative changes in transcript abundances of inflammasome pathway genes in retinas. Mean fold change vs. naïve ± SE, * p < 0.05, n = 3–5. ( C ) Intravitreal IL1-β measured at 6, 12, and 24 h after spOHT (red) and stOHT eyes (OD, blue). Mean ± SE, * p < 0.05, n = 5. Negative control: Casp1 −/− eyes (Cs1KO). ( D ) Quantification for HIF1a fluorescent labeling in retina wholemounts from eyes exposed to sham, stOHT, spOHT, or IR. Mean percentile change vs. sham ± SE, ** p < 0.05, n = 5.
Chemical Compound, Drug Methocult Gf, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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peptide, recombinant protein pam3cysk4  (EMC microcollections GmbH)
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EMC microcollections GmbH peptide, recombinant protein pam3cysk4
Repetitive IOP spikes induce the activation of inflammatory biomarkers and RGC dysfunction and loss. ( A ) Experimental paradigm of the spiking OHT (spOHS) model and steady OHT (stOHT) control; ( B ) qRT-PCR data on relative changes in transcript abundances of inflammasome pathway genes in retinas. Mean fold change vs. naïve ± SE, * p < 0.05, n = 3–5. ( C ) Intravitreal IL1-β measured at 6, 12, and 24 h after spOHT (red) and stOHT eyes (OD, blue). Mean ± SE, * p < 0.05, n = 5. Negative control: Casp1 −/− eyes (Cs1KO). ( D ) Quantification for HIF1a fluorescent labeling in retina wholemounts from eyes exposed to sham, stOHT, spOHT, or IR. Mean percentile change vs. sham ± SE, ** p < 0.05, n = 5.
Peptide, Recombinant Protein Pam3cysk4, supplied by EMC microcollections GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chemical compound, drug gentamycin  (Centrafarm Nederland B V)
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Centrafarm Nederland B V chemical compound, drug gentamycin
Repetitive IOP spikes induce the activation of inflammatory biomarkers and RGC dysfunction and loss. ( A ) Experimental paradigm of the spiking OHT (spOHS) model and steady OHT (stOHT) control; ( B ) qRT-PCR data on relative changes in transcript abundances of inflammasome pathway genes in retinas. Mean fold change vs. naïve ± SE, * p < 0.05, n = 3–5. ( C ) Intravitreal IL1-β measured at 6, 12, and 24 h after spOHT (red) and stOHT eyes (OD, blue). Mean ± SE, * p < 0.05, n = 5. Negative control: Casp1 −/− eyes (Cs1KO). ( D ) Quantification for HIF1a fluorescent labeling in retina wholemounts from eyes exposed to sham, stOHT, spOHT, or IR. Mean percentile change vs. sham ± SE, ** p < 0.05, n = 5.
Chemical Compound, Drug Gentamycin, supplied by Centrafarm Nederland B V, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody mouse monoclonal cd34 apc a750 beckman coulter  (Danaher Inc)
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Danaher Inc antibody mouse monoclonal cd34 apc a750 beckman coulter
Repetitive IOP spikes induce the activation of inflammatory biomarkers and RGC dysfunction and loss. ( A ) Experimental paradigm of the spiking OHT (spOHS) model and steady OHT (stOHT) control; ( B ) qRT-PCR data on relative changes in transcript abundances of inflammasome pathway genes in retinas. Mean fold change vs. naïve ± SE, * p < 0.05, n = 3–5. ( C ) Intravitreal IL1-β measured at 6, 12, and 24 h after spOHT (red) and stOHT eyes (OD, blue). Mean ± SE, * p < 0.05, n = 5. Negative control: Casp1 −/− eyes (Cs1KO). ( D ) Quantification for HIF1a fluorescent labeling in retina wholemounts from eyes exposed to sham, stOHT, spOHT, or IR. Mean percentile change vs. sham ± SE, ** p < 0.05, n = 5.
Antibody Mouse Monoclonal Cd34 Apc A750 Beckman Coulter, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Repetitive IOP spikes induce the activation of inflammatory biomarkers and RGC dysfunction and loss. ( A ) Experimental paradigm of the spiking OHT (spOHS) model and steady OHT (stOHT) control; ( B ) qRT-PCR data on relative changes in transcript abundances of inflammasome pathway genes in retinas. Mean fold change vs. naïve ± SE, * p < 0.05, n = 3–5. ( C ) Intravitreal IL1-β measured at 6, 12, and 24 h after spOHT (red) and stOHT eyes (OD, blue). Mean ± SE, * p < 0.05, n = 5. Negative control: Casp1 −/− eyes (Cs1KO). ( D ) Quantification for HIF1a fluorescent labeling in retina wholemounts from eyes exposed to sham, stOHT, spOHT, or IR. Mean percentile change vs. sham ± SE, ** p < 0.05, n = 5.

Journal: Cells

Article Title: The Inflammasome-Dependent Dysfunction and Death of Retinal Ganglion Cells after Repetitive Intraocular Pressure Spikes

doi: 10.3390/cells12222626

Figure Lengend Snippet: Repetitive IOP spikes induce the activation of inflammatory biomarkers and RGC dysfunction and loss. ( A ) Experimental paradigm of the spiking OHT (spOHS) model and steady OHT (stOHT) control; ( B ) qRT-PCR data on relative changes in transcript abundances of inflammasome pathway genes in retinas. Mean fold change vs. naïve ± SE, * p < 0.05, n = 3–5. ( C ) Intravitreal IL1-β measured at 6, 12, and 24 h after spOHT (red) and stOHT eyes (OD, blue). Mean ± SE, * p < 0.05, n = 5. Negative control: Casp1 −/− eyes (Cs1KO). ( D ) Quantification for HIF1a fluorescent labeling in retina wholemounts from eyes exposed to sham, stOHT, spOHT, or IR. Mean percentile change vs. sham ± SE, ** p < 0.05, n = 5.

Article Snippet: ELISA kits for mouse IL-1β (R&D ID# MBL00C) or Ella SimplePlex for IL1β, TNFα, and MCP1/CCL2 (Protein Simple, Bio-Techne Inc., Minneapolis, MN, USA), as described in [ ], were used to measure the cytokines released into the vitreous.

Techniques: Activation Assay, Quantitative RT-PCR, Negative Control, Labeling

Pro-inflammatory cytokines in the spOHT-challenged retina. Immunolabeling for IL-1β (blue) co-localized with neurons in the GCL and INL; TNFα-specific labelling (green) confined to GFAP + astroglia (arrows), infiltrating monocytes (arrowheads) in the retina 48 h after the spOHT challenge. ASC-citrine specks (magenta) co-localized with the GFAP + TNFα + astrocytes and Muller glia (yellow arrows) in the inner retina, and with astrocytes in the optic nerve. In control stOHT-treated retinas (bottom panels), both TNFα- and IL1β-specific labeling were reduced; no infiltrating monocytes were detected. Size bars in µm.

Journal: Cells

Article Title: The Inflammasome-Dependent Dysfunction and Death of Retinal Ganglion Cells after Repetitive Intraocular Pressure Spikes

doi: 10.3390/cells12222626

Figure Lengend Snippet: Pro-inflammatory cytokines in the spOHT-challenged retina. Immunolabeling for IL-1β (blue) co-localized with neurons in the GCL and INL; TNFα-specific labelling (green) confined to GFAP + astroglia (arrows), infiltrating monocytes (arrowheads) in the retina 48 h after the spOHT challenge. ASC-citrine specks (magenta) co-localized with the GFAP + TNFα + astrocytes and Muller glia (yellow arrows) in the inner retina, and with astrocytes in the optic nerve. In control stOHT-treated retinas (bottom panels), both TNFα- and IL1β-specific labeling were reduced; no infiltrating monocytes were detected. Size bars in µm.

Article Snippet: ELISA kits for mouse IL-1β (R&D ID# MBL00C) or Ella SimplePlex for IL1β, TNFα, and MCP1/CCL2 (Protein Simple, Bio-Techne Inc., Minneapolis, MN, USA), as described in [ ], were used to measure the cytokines released into the vitreous.

Techniques: Immunolabeling, Labeling